Methylthioninium Chloride BP Ph Eur Methylene Blue USP Manufacturers

Supplier, Manufacturer, Exporter of Methylene Blue or Methylthioninium Chloride, Muby Chemicals of Mubychem Group, established in 1976, is the original manufacturers of Specialty Chemicals, Pharmaceutical Excipient, Fragrance Food & Flavor chemicals, Reagent Grade Chemicals, Shale Gas Fracturing Chemicals in India. Mubychem Group has several manufacturing facilities spread across Western India and world wide contacts and toll manufacturers. We are exporting globally to countries like USA, Canada, Europe, UAE, South Africa, Tanzania, Kenya, Egypt, Nigeria, Cameroon, Uganda, Turkey, Mexico, Brazil, Chile, Argentina, Dubai, Korea, Vietnam, Thailand, Malaysia, Indonesia, Australia, China, Germany, France, Italy, Portugal etc.

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Methylene Blue or Methylthioninium Chloride CAS Number Trihydrate 61-73-4 for Anhydrous and 7220-79-3 for Trihydrate, EC EINECS 200-515-2, HS Code 32041351*,Molecular Weight 373.9, Chemical Formula: C16H18ClN3S-3H2O

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Methylene Blue
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Methylene Blue

Specifications of Methylene Blue are as follows:
Chemical Formula: C16H18N3CIS
Molecular Weight: 319.85 gm / mole (w/o water) 373.9 with water of crystallization
Chemical Name: 3,7-bis(Dimethylamino)-phenazathionium chloride Tetramethylthionine chloride
CAS Number: 61-73-4
C.I No.: 52015
Appearance: Dark Green Crystalline Powder
Assay (as is basis): 80 % - 85%
Assay (on dry basis): 98% -101%
Loss on drying: 10% -20%
Solubility: Clear Blue Solution
Ash content: 1% Maximum.
Residual Solvent: Nil
Aluminium: 100 ppm Maximum.
Cadmium: 1ppm Maximum.
Chromium: 10ppm Maximum.
Copper: 50ppm Maximum.
Tin: 10 ppm Maximum.
Iron: 100 ppm Maximum.
Manganese: 10ppm Maximum.
Mercury: Nil
Molybdenum: 10ppm Maximum.

Methylene Blue USP Grade Specifications

Methylene Blue
C16H18ClN3S-3H2O -- 373.90
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate.
C.I. Basic Blue 9 trihydrate [CAS 7220-79-3]
Anhydrous 319.86 [CAS 61-73-4]

Methylene Blue contains not less than 98.0 percent and not more than 103.0 percent of C16H18ClN3S, calculated on the dried basis.
Identification: Infrared Absorption 197K .

Loss on drying: Dry it at 75 and at a pressure not exceeding 5 mm of mercury for 4 hours: it loses between 8.0% and 18.0% of its weight.
Residue on ignition: not more than 1.2%.
Arsenic: Prepare the Test Preparation by mixing 0.375 g with 10 mL of water in the arsine generator flask. Add 15 mL of nitric acid and 5 mL of perchloric acid, mix, and heat cautiously to the production of strong fumes of perchloric acid. Cool, wash down the sides of the flask with water, and again heat to strong fumes. Again cool, wash down the sides of the flask, and heat to fumes. Cool, dilute with water to 52 mL, and add 3 mL of hydrochloric acid: the resulting solution meets the requirements of the test, the addition of 20 mL of 7 N sulfuric acid specified for Procedure being omitted. The limit is 8 ppm.
Copper or zinc: Ignite 1.0 g in a porcelain crucible, using as low a temperature as practicable, until all of the carbon is oxidized. Cool the residue, add 15 mL of 2 N nitric acid, and boil for 5 minutes. Filter the cooled solution, and wash any residue with 10 mL of water. To the combined filtrate and washing add an excess of 6 N ammonium hydroxide, and filter the solution into a 50 mL volumetric flask. Wash the precipitate with small portions of water, adding the washings to the filtrate, dilute the solution with water to volume, and mix. To 25 mL of the solution add 10 mL of hydrogen sulfide TS: no turbidity is produced within 5 minutes (absence of zinc). Any dark color produced does not exceed that of a control prepared by boiling a quantity of cupric sulfate, equivalent to 200 @g of copper, with 15 mL of 2 N nitric acid for 5 minutes and by treating this solution as directed above, beginning with “Filter the cooled solution” (0.02% of copper).
Chromatographic purity: To pass the test.
Assay: Transfer about 100 mg of Methylene Blue, accurately weighed, to a 250-mL volumetric flask, dissolve in and dilute with diluted alcohol to volume, and mix. Transfer 5.0 mL of this solution to a 100-mL volumetric flask, dilute with diluted alcohol to volume, and mix. Transfer 5.0 mL of this solution to a 50-mL volumetric flask, dilute with diluted alcohol to volume, and mix. This solution contains about 2 @g per mL. Dissolve an accurately weighed quantity of USP Methylene Blue RS in diluted alcohol, and dilute quantitatively and stepwise with diluted alcohol to obtain a Standard solution having a known concentration of about 2 @g per mL. Concomitantly determine the absorbance of both solutions in 1-cm cells at the wavelength of maximum absorbance at about 663 nm, with a suitable spectrophotometer, using diluted alcohol as the blank. Calculate the quantity, in mg, of C16H18ClN3S in the Methylene Blue taken by the formula:

50C(AU / AS)

in which C is the concentration, in @g per mL, of anhydrous methylene blue in the Standard solution; and AU and AS are the absorbance of the solution of Methylene Blue and the Standard solution, respectively. 

Methylthioninium Chloride BP or Methylthioninium Chloride Ph. Eur is also offered.

IUPAC name is 3,7-Bis(dimethylamino)phenothiazin-5-ylium chloride or 3,7-bis(Dimethylamino)-phenothiazin-5-ium chloride

Manufacturers:

MUBY CHEMICALS
Ambernath Mumbai, Ankleshwar Gujarat, India
TEL: (OFFICE) +912223770100, +912223726950
Current Date Time in India GMT+5:30

e-mail: info@mubychem.com

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Call toll-free 1-844-682-9243 (1-844-MUBYCHEM)

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Last 27 August, 2022

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Methylene Blue is also called ( CAS No. 548-62-9 and 603-47-4 )
Basic blue 9 (C.I. 52015)
3,7-Bis-(dimethylamino)-phenazathonium chloride
3,7-Bis-(dimethylamino)-5-phenothiazinium chloride
C.I. 52015
Methylene blue (C.I. 52015)
Methylthionine chloride
Solvent blue 8 (C.I. 52015)
Swiss blue
Tetramethylthionine chloride
Urolene blue